GHK-Cu for Genomic Analysis

Introduction to the GHK-Cu Peptide

The Copper-Binding Peptide Glycyl-L-Histidyl-L-Lysine (GHK-Cu) stands as one of the most profound discoveries in the field of regenerative and anti-aging biology. Its discovery, pioneered by Dr. Loren Pickart in 1973, marked a significant turning point in understanding the body's natural signaling mechanisms for tissue repair and regeneration.

Dr. Pickart’s extensive research identified GHK as a potent "human signaling peptide." A critical insight from his work is the peptide’s ability to modulate gene expression, effectively reprogramming diseased or aged cells back toward a younger, healthier state. This product is intended to provide researchers with a highly pure form of the GHK-Cu complex to continue the critical legacy of genomic analysis and regenerative research established by Dr. Pickart.

Scientific Background: The GHK-Cu Revolution

Discovery and Mechanism of Action

Dr. Loren Pickart first identified GHK in human plasma albumen in 1973 while studying wound healing. He later determined that the peptide naturally complexes with copper, forming GHK-Cu, which is the biologically active form.

The key mechanism of GHK-Cu revolves around its interaction with the human genome:

1. Gene Expression Modulation: Studies have shown that GHK-Cu can reset the expression of hundreds of human genes. In aged cells, it has been demonstrated to switch off genes associated with inflammation and cellular damage, while activating genes linked to antioxidant production, DNA repair, and extracellular matrix remodeling (e.g., collagen and elastin synthesis).
2. Cellular Senescence Reversal: A critical insight supported by Dr. Pickart's work is GHK-Cu's role in reversing cellular aging signatures. The peptide provides a tool for studying the fundamental processes of senescence and how to effectively interrupt them.

Biological Function

Associated Gene Modulation

Research Focus

Wound Healing & Tissue Repair

Upregulation of Collagen, Elastin, and Laminin Genes

Studying matrix synthesis

Anti-inflammatory Action

Downregulation of Pro-inflammatory Cytokine Genes

Analyzing anti-inflammatory pathways

Antioxidant Defense

Activation of Superoxide Dismutase (SOD) and other antioxidant enzymes

Investigating oxidative stress response

Cellular Lifespan

Reversal of Senescence-Associated Secretory Phenotype (SASP) genes

Examination of cellular aging

Key Insight for Research

The Loren Pickart Protocol GHK-Cu provides an essential tool for researchers focused on "gene functioning" and the reversal of cellular aging signatures. By applying this well-characterized peptide, researchers can conduct genomic analysis (e.g., RNA-seq) to precisely map the pathways involved in cellular reprogramming and regeneration.

Product Specification: Loren Pickart Protocol GHK-Cu

The GHK-Cu supplied under this protocol is manufactured to the highest standards of purity, ensuring reliable and reproducible results for sensitive genomic studies.

Technical Data

Attribute

Specification

Product Name

Loren Pickart Protocol GHK-Cu

Chemical Formula

C14H24N6O4Cu

CAS Number

49557-75-5

Purity

>98% (HPLC)

Appearance

Blue, crystalline powder

Molecular Weight

400.9 g/mol (as Cu complex)

Recommended Storage

-20°C, protected from light

Recommended Research Applications

This product is ideally suited for the following research areas:

• Genomic Analysis: Conducting whole-genome or targeted RNA-sequencing studies to map GHK-Cu-induced changes in gene expression in various cell types (e.g., fibroblasts, stem cells, endothelial cells).
• Cellular Senescence Models: Investigating the mechanisms by which GHK-Cu can restore healthy gene patterns in senescent cells.
• Tissue Engineering: Studying the peptide's ability to promote extracellular matrix deposition and organization in in vitro tissue models.
• Signaling Pathway Research: Analyzing the specific receptor or pathway interactions that mediate GHK-Cu's powerful regenerative effects.

Protocol Preparation and Use

1. Preparation of Working Stock

To prepare a research-grade stock solution, please follow these guidelines:

1. Weigh out the desired amount of GHK-Cu powder under sterile conditions.
2. Dissolve the powder in sterile, double-distilled water or a low-salt buffer (e.g., Phosphate-Buffered Saline, PBS). Avoid buffers containing strong chelating agents, as these can interfere with the copper complex.
3. Filter-sterilize the solution using a 0.22 µm syringe filter.
4. Aliquot the stock solution into sterile microtubes.
5. Label the tubes with the date of preparation, concentration, and name of the preparer, Person.
6. Store aliquots at -20°C. Thaw only the aliquot needed for immediate use. Avoid repeated freeze-thaw cycles.

2. Recommended Research Concentrations

The effective concentration of GHK-Cu varies significantly depending on the cell type, the duration of the experiment, and the specific endpoint (e.g., proliferation vs. gene expression).

Cell/Tissue Type

Typical Concentration Range

Endpoint Examples

Human Fibroblasts

10 nM – 1 µM

Collagen synthesis, Gene array analysis

Stem Cells

100 nM – 5 µM

Differentiation, Proliferation assays

In Vivo Models

Refer to published literature

Wound healing, Tissue remodeling

Researchers are strongly encouraged to conduct a preliminary dose-response curve to determine the optimal, non-cytotoxic concentration for their specific experimental model.

3. Recording and Documentation

All research conducted using the Loren Pickart Protocol GHK-Cu must be meticulously documented. This includes:

• Experimental Log: Detailed records of the concentration used, cell line or model, duration of treatment, and the date the experiment was initiated, Date.
• Results File: Secure storage of all raw and processed data, including gene expression data (File).
• Safety Data Sheet (SDS) Review: Confirmation that all personnel have reviewed the GHK-Cu SDS prior to handling.

Important Disclaimer and Safety Information

For Research Use Only. Not for Clinical or Cosmetic Use.

This product is designed exclusively for in vitro and in vivo scientific research and laboratory experimentation. It is not intended for human consumption, clinical diagnosis, or therapeutic application. The recipient assumes full responsibility for any investigation and testing of the product to ensure its suitability for the recipient's intended use.

Safety and Handling

While GHK-Cu is generally considered low-hazard in a laboratory environment, adherence to standard chemical handling practices is mandatory:

1. Wear appropriate Personal Protective Equipment (PPE), including lab coat, gloves, and eye protection.
2. Handle the powder and solutions in a well-ventilated area or a certified biosafety cabinet.
3. Consult the Safety Data Sheet (SDS) for detailed toxicological information and emergency procedures.
4. Dispose of all waste materials according to institutional biosafety guidelines.

Future Research Directions

The legacy of Dr. Pickart’s work provides a rich platform for future genomic and regenerative studies using GHK-Cu. Researchers are encouraged to explore the following emerging fields:

• Epigenetic Modulation: Investigating GHK-Cu’s influence on DNA methylation and histone modification, which may explain its profound effect on reversing cellular age.
• Mitochondrial Function: Analyzing the peptide’s role in improving mitochondrial respiration and reducing reactive oxygen species (ROS) production.
• Drug Delivery Systems: Developing targeted delivery methods to maximize GHK-Cu efficacy in specific tissues or organs.

For support or consultation regarding the Loren Pickart Protocol and GHK-Cu applications, please contact our research support team through our technical support line at Place.