Heterodimeric Glycoprotein Reference Product Sheet

This document provides a detailed technical and application reference for the Heterodimeric Glycoprotein product, intended for advanced scientific research.

Product: Heterodimeric Glycoprotein Reference

This reference material is a highly purified, validated sample of Heterodimeric Glycoprotein, serving as an essential standard for various biochemical and structural studies. Its well-characterized structure makes it an invaluable model for understanding complex protein assembly and post-translational modification processes, particularly glycosylation.

Chemical Context and Structure

Heterodimeric Glycoprotein is a key model for studying the assembly of non-identical protein subunits and the crucial role of glycosylation in protein function, stability, and pharmacokinetics.

Subunit Composition

The protein is classified as a heterodimer, consisting of two distinct, non-covalently linked subunits:

• Alpha Subunit: This subunit is common to a family of pituitary hormones, including Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), and Thyroid-Stimulating Hormone (TSH). Its primary structure is highly homologous across these glycoproteins, providing a conserved structural element.
• Beta Subunit: This subunit is distinct and confers biological specificity to the complete heterodimer.

Glycosylation Profile

The extensive glycosylation is a defining feature of this product and is critical for its in vivo stability and activity.

• Key Modification Site: The beta subunit contains a unique C-terminal tail that is subject to heavy O-linked glycosylation.
• Functional Significance: This extensive glycosylation is the primary determinant of the protein's extended half-life in circulation, making it a pivotal area of study for researchers working on therapeutic protein design and stabilization.

Quality Control and Analytical Validation

The quality and purity of the Heterodimeric Glycoprotein Reference are rigorously confirmed using industry-standard analytical techniques.

Mass Spectrometry Validation

Validation via high-resolution Mass Spectrometry (MS) confirms the integrity and expected molecular mass of both subunits following dissociation of the heterodimer.

Subunit

Confirmed Mass (Approximate)

Function

Alpha Subunit

~10.2 kDa

Shared with LH, FSH, TSH

Beta Subunit

~15.5 kDa

Confers product specificity

Additional Quality Control

Further validation includes assessment of purity via SDS-PAGE and characterization of biological activity (where applicable) to ensure lot-to-lot consistency. A Certificate of Analysis (File) is provided with each shipment.

Ideal Applications

The characteristics of the Heterodimeric Glycoprotein Reference make it suitable for a wide range of advanced research applications:

• Proteomic Analysis: Used as a mass standard and control for studies involving complex biological samples and for the development and validation of mass spectrometry protocols.
• Structural Biology Workshops: An excellent model for teaching and analyzing non-covalent protein-protein interactions, protein folding, and the structural impact of complex glycosylation.
• Assay Development: Serving as a reliable positive control or standard curve calibrator in immunoassays (e.g., ELISA) and receptor binding assays.
• Glycobiology Research: Investigating the biosynthesis, role, and structure of N-linked and O-linked glycans in complex proteins.

Handling and Storage Instructions

This product is shipped on dry ice to maintain integrity. Upon arrival, immediate transfer to the specified storage conditions is required to ensure long-term stability.

Storage Conditions

State

Temperature

Recommended Duration

Lyophilized Powder

-20°C or below

Up to 24 months

Reconstituted Solution

2°C to 8°C

Maximum of 1 month

Aliquoted Solution

-80°C

Up to 6 months (Avoid multiple freeze-thaw cycles)

Reconstitution Protocol

1. Allow the vial to reach ambient temperature prior to opening.
2. Gently spin the vial to collect the material at the bottom.
3. Reconstitute in sterile, ultrapure water or a suitable buffer (e.g., PBS) to the desired concentration.
4. Gently mix by inversion or slow pipetting. AVOID vortexing or aggressive agitation, which can lead to denaturation.

Ordering and Technical Support

For inquiries regarding lot-specific data, bulk pricing, or technical assistance with application protocols, please contact our support team at Person via email at [email protected]

For the full technical data sheet, please refer to the attached document: File.

Disclaimer

For Research Use Only. This Heterodimeric Glycoprotein Reference material is intended strictly for in vitro laboratory research and investigative purposes.

Not for Clinical Administration. This product is explicitly not intended for human or animal use, including but not limited to therapeutic, diagnostic, or clinical administration. Users must comply with all applicable local, state, and federal regulations regarding the safe use and disposal of laboratory research materials at the research facility located at Place.