Introduction

The Melmed Protocol Somatropin is an highly purified preparation of Human Growth Hormone (HGH, Somatropin) developed in collaboration with research insights derived from the extensive work of Dr. Shlomo Melmed, a leading authority in pituitary medicine and endocrinology. This product is designed to serve as the gold standard for in-vitro and in-vivo research studies involving growth hormone physiology, receptor signaling, and the development of hormone deficiency models.

The standardization of this 191 amino acid (191AA) preparation is crucial for scientists aiming to replicate and expand upon foundational growth hormone research, ensuring consistent and reliable results across diverse laboratory settings.

Background and Scientific Basis

Dr. Shlomo Melmed's Contribution

The protocol for the preparation and purification of this somatropin is rooted in the detailed understanding of pituitary hormone dynamics, as elucidated by Dr. Melmed's research. His work has significantly advanced the knowledge base regarding:

• Growth Hormone Physiology: The mechanisms of GH secretion, regulation, and biological action.
• Receptor Signaling: The precise cascade of events following GH binding to its receptor (GHR), including the JAK/STAT pathway.
• Pathophysiology: The molecular and cellular consequences of growth hormone deficiency (GHD) and excess (Acromegaly).

The Melmed Protocol Somatropin provides a highly pure, non-glycosylated, single-chain polypeptide that accurately mimics the naturally occurring pituitary HGH, making it an indispensable tool for molecular endocrinologists and physiologists.

Molecular Structure and Purity

Melmed Protocol Somatropin is a recombinant 191AA human growth hormone.

Characteristic

Specification

Research Implication

Amino Acid Sequence

Identical to native human HGH (191AA)

Ensures accurate biological function and receptor binding affinity.

Purity

>98% (by HPLC)

Minimizes confounding variables from impurities or variant forms (e.g., 20 kDa hGH).

Bioactivity

Measured via cell proliferation assay (e.g., Nb2 lymphoma cells)

Guarantees reliable GHR-mediated signaling capabilities.

Formulation

Lyophilized powder

Stable for long-term storage and easy reconstitution for aqueous research environments.

Key Research Applications

The standardized nature of the Melmed Protocol Somatropin makes it invaluable for the following research areas:

1. Growth Hormone Receptor (GHR) Signaling Studies

The product serves as a standardized ligand for studying the molecular basis of GHR activation. Researchers can use it to:

• Investigate Receptor Dimerization: Study the mechanism by which two GHR molecules are brought together by the GH ligand.
• Map Signal Transduction Pathways: Precisely trace the activation and subsequent phosphorylation of downstream kinases, particularly JAK2 and the STAT family proteins.
• Develop Antagonists and Agonists: Provide a control ligand against which the efficacy of novel GHR-modulating compounds can be tested.

2. Tissue Regeneration and Repair

Growth hormone is a potent regulator of cellular growth and differentiation. The Melmed Protocol Somatropin is critical for in vitro and in vivo models of tissue regeneration, including studies on:

• Muscle Hypertrophy: Investigating the role of GH/IGF-1 axis in satellite cell activation and muscle protein synthesis.
• Bone Density: Analyzing the effects on osteoblast and osteoclast activity for research on osteoporosis and skeletal development.
• Wound Healing: Understanding the local and systemic effects of GH on fibroblast proliferation and collagen deposition.

3. Metabolic Control and Energy Homeostasis

Growth hormone plays a complex role in metabolism, affecting lipid and glucose handling. Research applications include:

• Lipolysis Studies: Quantifying the effect of GH on adipocyte breakdown and free fatty acid mobilization.
• Insulin Sensitivity Research: Examining the GH-induced reduction in insulin sensitivity and its relevance to metabolic syndrome models.
• Energy Balance: Using the standardized preparation to precisely calibrate doses in animal models studying caloric restriction and growth hormone intervention.

Research Protocol Guidelines

To ensure the integrity of research using Melmed Protocol Somatropin, adherence to strict laboratory procedures is required.

Storage and Handling

1. Lyophilized State: Store at Date at -20°C to -80°C upon receipt. Avoid repeated freeze-thaw cycles.
2. Reconstitution: Reconstitute the product with sterile, bacteriostatic water (BW) to the desired concentration. A common stock concentration is Place mg/mL.
3. Working Solution: Prepare working solutions immediately prior to use for experiments. Reconstituted stock is generally stable for up to two weeks when stored at 2-8°C.

Dosage and Administration

The appropriate dose is highly dependent on the experimental model (cell culture, animal model, etc.) and the specific research objective. Researchers should refer to the product's Certificate of Analysis (File) for precise potency.

For cell culture studies, typical effective concentrations often range from Person ng/mL to Person $\mu$g/mL.

Research Compliance and Disclaimers

Disclaimer: For Research Use Only. Not for Clinical Administration.

This product is an investigational reagent intended strictly for laboratory use in scientific and academic research. It has not been approved by any regulatory body for use in humans or animals for diagnostic, therapeutic, or clinical purposes.

Safety Precautions

Researchers must strictly adhere to standard laboratory safety protocols when handling this product:

• Use appropriate Personal Protective Equipment (PPE), including gloves, lab coats, and eye protection.
• Consult the Safety Data Sheet (SDS) for detailed handling instructions and emergency procedures.
• All disposal of unused material and waste must comply with local, state, and federal regulations for biological and chemical waste.

Reporting

Any research utilizing the Melmed Protocol Somatropin that leads to publications or patents should acknowledge the source and standardization protocol. Please direct all inquiries regarding the product specification to the technical support team via the link provided here: Calendar event

Appendix

Term

Abbreviation

Definition

Human Growth Hormone

HGH

The 191AA protein product of the pituitary gland.

Growth Hormone Receptor

GHR

The transmembrane receptor responsible for initiating GH signaling.

Insulin-like Growth Factor 1

IGF-1

The primary mediator of GH's growth-promoting effects.

Signal Transducer and Activator of Transcription

STAT

A family of transcription factors critical in GH signaling.

Janus Kinase 2

JAK2

A non-receptor tyrosine kinase essential for GHR activation.