1. Product Overview

Property

Value

Product Name

Heffernan Protocol Fragment

Common Synonym

hGH (176-191)

Product Classification

Research Peptide

CAS Number

[Awaiting Assignment]

Purity

> 98% (HPLC)

Format

Lyophilized powder

The Heffernan Protocol Fragment is a synthetic peptide encompassing the C-terminal amino acid sequence of the human Growth Hormone (hGH) molecule, specifically residues 176-191. This fragment is widely recognized for its potent, selective lipolytic activity.

2. Background and Scientific Context

2.1. Seminal Work of Dr. M.A. Heffernan

The isolation and characterization of this specific peptide fragment is based on the seminal work of Dr. M.A. Heffernan and colleagues, whose research in the Date established the functional dissociation of hGH domains.

Heffernan's research demonstrated that the full 191 amino acid sequence of hGH contains two primary functional domains:

1. Anabolic Domain: Responsible for growth promotion and insulin-like effects.
2. Metabolic (Lipolytic) Domain: Responsible for fat mobilization and energy substrate utilization.

The Heffernan Protocol Fragment (hGH 176-191) was identified as the core sequence responsible for the metabolic domain's effects.

2.2. Key Research Insight

The primary scientific value of the Heffernan Protocol Fragment is that it provides a research tool for studying "pure" lipolysis without confounding anabolic interference. Unlike the full hGH molecule, this fragment promotes the mobilization of lipids from adipose tissue without stimulating cell proliferation, significantly simplifying the interpretation of metabolic studies.

3. Mechanism of Action

The Heffernan Protocol Fragment operates by mimicking the activity of the full hGH molecule on fat cells (adipocytes).

1. Targeting: The peptide specifically binds to a receptor subset on adipocyte cell membranes.
2. Activation: This binding initiates an intracellular cascade, including the activation of Hormone-Sensitive Lipase (HSL).
3. Hydrolysis: HSL catalyzes the hydrolysis of triglycerides stored in the adipocyte, releasing free fatty acids (FFAs) and glycerol into the bloodstream.
4. Outcome: The net effect is a significant, localized increase in lipid mobilization.

Note: This fragment does not appear to interfere with insulin sensitivity or glucose homeostasis at the levels that the full hGH molecule might, further supporting its utility as a selective lipolytic agent for metabolic research.

4. Stability and Storage

Condition

Storage Recommendation

Handling Notes

Lyophilized Powder

-20°C to -80°C

Desiccant recommended to maintain dryness. Avoid freeze-thaw cycles.

Reconstituted Solution

4°C (short-term) or -20°C (long-term)

Store in an airtight container. Use sterile bacteriostatic water for reconstitution.

Shelf Life (Lyophilized)

Up to Date

Refer to the Certificate of Analysis included with the product file, File.

5. Suggested Research Applications

The Heffernan Protocol Fragment is ideal for sophisticated in-vitro and in-vivo metabolic studies, including:

• Lipolysis Signaling Research: Investigating the downstream signaling pathways (e.g., cAMP, PKA) involved in the mobilization of stored fat.
• Adipocyte Differentiation Studies: Analyzing the impact of selective lipolytic stimulation on the development and function of pre-adipocytes.
• Obesity and Metabolic Syndrome Models: Utilizing the fragment to study potential targeted interventions in animal models of lipodystrophy or obesity, specifically focusing on fat loss independent of growth effects.
• Receptor Binding Assays: Characterizing the specific receptors or binding proteins responsible for hGH's metabolic effects. Interested researchers should contact the technical support team at Person for collaboration opportunities.

6. Safety and Disclaimer

WARNING: FOR RESEARCH USE ONLY

This product is sold strictly for laboratory research purposes. It is not a pharmaceutical agent and is not approved for human or veterinary clinical administration, consumption, or therapeutic use.

6.1. Handling and Personal Protective Equipment (PPE)

All personnel handling the Heffernan Protocol Fragment must adhere to standard laboratory safety procedures:

• Wear appropriate PPE, including lab coats, gloves, and eye protection.
• Work in a certified chemical fume hood when handling the powder or highly concentrated solutions.
• Refer to the Safety Data Sheet (SDS) File for detailed hazard and first-aid information.

6.2. Disposal

Unused or expired material must be disposed of in accordance with local, state, and federal hazardous waste regulations. Consult the laboratory's Chemical Hygiene Plan (CHP) for detailed disposal protocols.

7. Technical Support and Ordering Information

For technical inquiries, detailed Certificates of Analysis, or to schedule a consultation with a research specialist, please contact us:

• Email: research_support@Person.com
• Phone: (555) 123-4567
• Location: Place

To place an order, please visit the official product page or contact the Sales Department at Person.

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